Autoradiography of cells double labeled with H-3 and C-14.

Preparation of 14 C-labeled showdomycin.

When Streptomyces shoivdoensis (N2-209-56) mycelia were grown in the presence of sodium acetate-2-uC, the radioactive carbon from acetate-2-uC was fairly well incorporated into the showdomycin molecule ; with approximately 14 % of the total radioactivity of the added sodium acetate-2-14C being incorporated during a 10-hour incubation. Although three antibiotic substances were detected in the fe...

Class I HDAC imaging using [ (3)H]CI-994 autoradiography.

[ (3)H]CI-994, a radioactive isotopologue of the benzamide CI-994, a class I histone deacetylase inhibitor (HDACi), was evaluated as an autoradiography probe for ex vivo labeling and localizing of class I HDAC (isoforms 1-3) in the rodent brain. After protocol optimization, up to 80% of total binding was attributed to specific binding. Notably, like other benzamide HDACi, [ (3)H]CI-994 exhibits...

S - Radiolysis of Crystalline 14 C - Labeled Amino Acids

Synthesis of ring labeled [ 1 ’ - 14 C ] - L - tyrosine Ryszard

The enzyme β-tyrosinase (tyrosine phenol lyase, EC 4.1.99.2) has been shown [8, 9, 15] to catalyze the decomposition of L-Tyr to phenol, pyruvate and ammonia. Under some conditions, this enzyme also participates in the reverse reaction leading to formation [10, 12, 13] of L-Tyr (Scheme 1). As a metabolism of L-Tyr is an important process of the living cells, the mechanism of the above reaction ...

Urinary metabolites of 14 C-labeled thyroxine in man.

Studies were carried out to determine the chemical structures of thyroxine metabolites after total deiodination. Normal subjects were given thyroxine labeled with (14)C on the nonphenolic ring and the alanine side chain, 8-11 mug/day for 10 days. By paper chromatography of fresh urine, six or more (14)C-labeled compounds were separated. The (14)C-labeled metabolites were concentrated by passing...